Quantification of WT1 mRNA by Competitive NASBA in AML Patients.
نویسندگان
چکیده
منابع مشابه
The Prognostic Impact of WT1 Expression Levels, Mutations, and SNP rs16754 in AML Patients: A Retrospective Cohort Study
Background and Objective: The clinical outcomes and treatment options for acute myeloid leukemia (AML) patients are highly dependent upon molecular markers. In this study, Wilms tumor (WT1) (exons 7 and 9) mutations, SNP rs16754, and WT1 expression levels in 130 random AML patients were screened; FMs-like tyrosine kinase-3 internal tandem duplication (FLT3-ITD), nucleophosmin (NPM1), and CCAAT/...
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to 40 nM is able to largely overcome the inhibi-tory impact of a 2.5-to 5.0-M concentration of EGCG. These considerations argue against the possibility that even high levels of EGCG inges-tion would impact upon the clinical efficacy of bortezomib. Nonetheless, these data serve as an always timely reminder for healthcare providers of the importance of eliciting a complete history from patients ...
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The goal of the present study was to develop a competitive PCR assay to measure changes in the expression of endothelial nitric oxide synthase (eNOS) mRNA levels throughout the canine vascular tree. A partial sequence of canine eNOS cDNA (1.86 kb), inducible NOS (1.95 kb), and neuronal NOS (1.16 kb) was cultured from canine aortic endothelial cells, LPS-treated canine splenic vein endothelial c...
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The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...
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ژورنال
عنوان ژورنال: The Kurume Medical Journal
سال: 2001
ISSN: 0023-5679,1881-2090
DOI: 10.2739/kurumemedj.48.129